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Wednesday, July 29, 2020 | History

2 edition of rat bone marrow stromal cell osteogenic system found in the catalog.

rat bone marrow stromal cell osteogenic system

Alexandra Lenore Herbertson

rat bone marrow stromal cell osteogenic system

characterization of subpopulations, fractionation and effects of PDGF

by Alexandra Lenore Herbertson

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  • 34 Currently reading

Published by University of Toronto, Faculty of Dentistry] in [Toronto .
Written in English


Edition Notes

Thesis (Ph.D.)--University of Toronto, 1998.

Statementby Alexandra Lenore Herbertson.
The Physical Object
Paginationix, 125 leaves.
Number of Pages125
ID Numbers
Open LibraryOL17469578M
ISBN 10061233905X

The differentiation of adipocytic and osteogenic cells has been investigated in cultures of adult rat marrow stromal cells. Adipocytic differentiation was assessed using morphological criteria, changes in expression of procollagen mRNAs, consistent with a switch from the synthesis of predominantly fibrillar (types I and III) to basement membrane (type IV) collagen, and the induction of Osteogenic differentiation potential of umbilical cord-derived mesenchymal stromal cells (UC-MSCs) in comparison to bone marrow-derived mesenchymal stromal cells (BM-MSCs). (A) The negative result for Alizarin Red staining of BM-MSCs cultured in DMEM supplemented with

Abstract. Human skeletal stem cells (also known as bone marrow-derived stromal cells, hBMSCs) are adult clonogenic cells located in a perivascular niche in the bone marrow. hBMSCs are capable of multilineage differentiation into various mesoderm-cell types including bone forming osteoblastic :// Osteoblastic and chondroblastic (i.e., osteogenic) cells belong to the stromal cell system, which is associated with bone marrow, and bone and is separate from the hematopoietic stem-cell ://

Bone marrow-derived stem cells Osteogenic differentiation () Adult rat and human bone marrow stromal cells differentiate into neurons. J Neurosci Res – PubMed Toh YC, Kim SH, Foo HL, Tan CH, van Noort D, Park S, Yu H () A gel-free 3D microfluidic cell culture system. Biomaterials – PubMed Google Scholar. Osteogenic differentiation of rat bone marrow stromal cells cultured on Arg–Gly–Asp modified hydrogels without dexamethasone and β-glycerol phosphate Article in Biomaterials 26(17)


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Rat bone marrow stromal cell osteogenic system by Alexandra Lenore Herbertson Download PDF EPUB FB2

THE RAT BONE MARROW STROMAL CELL OSTEOGENIC SYSTEM: CHARACTERIZATION OF SUBPOPULATIONS, FRACTIONATION, AND EFFECTS OF PDGF by Alexandra Lenore Herbertson Doctor of Philosophy, Graduate Department of Dentistry, University of Toronto ABSTRACT The objective of this thesis was to better define and characterize the   Bone marrow samples from rats long bones used for isolation of stromal cells (BMSCs).

Under determinate culture conditions BMSCs were differentiated in osteogenic cell lines detected by Alizarin red staining three weeks after isolation. BMSCs rat bone marrow stromal cell osteogenic system book autologue cells model showed high osteogenetic potential and calcification capatibility in ://   Osteogenic differentiation of rat bone marrow stromal cells cultured on Arg–Gly–Asp modified hydrogels without dexamethasone and β-glycerol phosphate Author links open overlay panel Heungsoo Shin a 1 Johnna S.

Temenoff a Gregory C. Bowden a Kyriacos Zygourakis b Mary C. Farach-Carson c Michael J. Yaszemski d Antonios G. Mikos a b A luminescent cell viability assay using RBMSCs was performed for screening cytotoxicity of the developed HA and SPCL scaffolds.

Results corroborated previous ones which have demonstrated in vitro, the superior performance of the HA and SPCL scaffolds on   Activation of GLP-1 Receptor Promotes Bone Marrow Stromal Cell Osteogenic Differentiation through β-Catenin Jingru Meng, 1, 5 Xue Ma, 1, 5 Ning Wang, 1, 5 Min Jia, 1 Long Bi, 2 Yunying Wang, 3 Mingkai Li, 1 Huinan Zhang, 1 Xiaoyan Xue, 1 Zheng Hou, 1 Ying Zhou, 1 Zhibin Yu, 3 Gonghao He, 4, 6 and Xiaoxing Luo 1, ∗ Bone marrow contains a colony-forming, fibroblast-like cell population called bone marrow mesenchymal stem cells or bone marrow stromal cells (BMSCs) [1, 2].Since BMSCs are capable of differentiating into multiple lineages (osteogenic, chondrogenic, adipogenic, neurogenic, and myogenic lineages), they have attracted significant interest as useful somatic stem cells for use in tissue Elisabeth H.

Javazon, David C. Colter, Emily J. Schwarz and Darwin J. Prockop, Rat Marrow Stromal Cells are More Sensitive to Plating Density and Expand More Rapidly from Single‐Cell‐Derived Colonies than Human Marrow Stromal Cells, STEM CELLS, 19, 3, (), ().

Why Functionally Verify Rat MSC Multipotency In Vitro?. Mesenchymal stem/stromal cells (MSCs) can be characterized based on the expression of specific cell surface markers, the absence of hematopoietic markers, and adherence to plastic in vitro. To more rigorously determine if a cell is truly an MSC, it is important to also verify its ability to differentiate into adipocytes, chondrocytes   Improved understanding of the interactions between bone cells and endothelial cells involved in osteogenesis should aid the development of new strategies for bone tissue engineering.

The aim of the present study was to determine whether direct communication between bone marrow stromal cells (MSC) and human umbilical vein endothelial cells (EC) could influence the osteogenic potential Whole body vibration (WBV), consisting of a low-magnitude, high-frequency (LMHF) signal, is anabolic to bone in vivo and may act through alteration of the lineage commitment of mesenchymal stromal cells (MSC).

We investigated the effect of LMHF vibration on rat bone marrow-derived MSCs (rMSCs) in an in vitro :// Mesenchymal stem/stromal cells (MSCs) are known to be useful for treating local bone diseases. However, it is not known if MSCs are effective for treating systemic bone diseases, as the risk for mortality following intravenous MSC administration has hindered research progress.

In this study, we compared the safety and efficacy of intra-bone marrow and intravenous administration of MSCs for the Stimulation of osteogenic protein expression for rat bone marrow stromal cells involved in the ERK signalling pathway by the ions released from Ca 7 Si 2 P 2 O 16 bioceramics X.

Zhang, C. Wu, J. Chang and J. Sun, J. ://   Kunkel, N. et al. Comparing the osteogenic potential of bone marrow and tendon-derived stromal cells to repair a critical-sized defect in the rat femur.

J Tissue Eng Regen Med. Identification of differentiation markers during transformation of stromal cells into osteoblasts in a time-dependent manner may be informative for cell-based tissue engineering. Therefore, we investigated the effects of osteogenic medium (OM) on the proliferation and differentiation of rat bone marrow stromal Bone tissue engineering is a potential pathway for bone regeneration, and bone marrow-derived MSCs are dominant seed cell sources for bone engineering.

MSCs possess the capability to differentiate into bone, cartilage, muscle and fat when supplied with nutrition and different types of growth factors (8).

Xia L, Xu Y, Chang Q, et al: Maxillary sinus floor elevation using BMP-2 and Nell-1 gene-modified bone marrow stromal cells and TCP in rabbits. Calcif Tissue Int.

– View Article: Google Scholar: PubMed/NCBI. 28 McCabe LR: Understanding the pathology and mechanisms of type 1 diabetic bone loss. J Cell Biochem. –   Churchman, S. M., Boxall, S. A., McGonagle, D. & Jones, E. Predicting the remaining lifespan and cultivation-related loss of osteogenic capacity of bone marrow multipotential stromal cells Rat bone marrow stromal cells comprise a heterogeneous mixture of cell lineages including osteoblastic cells.

When grown in the presence of ascorbic acid, beta-glycerophosphate and 10(-8) M dexamethasone, osteoprogenitor cells within the population divide and differentiate to form bone nodules (Maniatopoulos et al.,Cell Tissue Res., Effect of hypophysectomy on the proliferation and differentiation of rat bone marrow stromal cells (HX) rat as a model.

In the present study, we use an in vitro culture system to examine the effects of HX on the osteogenic potential of rat bone marrow stroma. With the intact animal as a control, we used [3H]thymidine incorporation and cell   We evaluated whether dexamethasone augments the osteogenic capability of bone marrow-derived stromal cells (BMSCs) and muscle tissue-derived stromal cells (MuSCs), both of which are thought to contribute to ectopic bone formation induced by bone morphogenetic protein-2 (BMP-2), and determined the underlying mechanisms.

Rat BMSCs and MuSCs were cultured in growth media with ?id=/. Rat bone marrow stromal cells were cultured in vitro. At days of culture, dense clusters of polygonal cells were formed, and they mineralized days later.

The cells resembling osteoblasts or young osteocytes were histologically observed to be embedded in mineralized or unmineralized extrace The effect of conditioned medium (CM) from rat calvaria (RC) cel cultures on the growth and differentiation of osteogenic cells in rat bone marrow stromal cell (BMSC) cultures was ://After physico-chemical scaffold characterization, rat bone marrow stromal cells were cultured on the composite scaffolds in maintenance medium and then in osteogenic medium.

Quantitative PCR, colorimetric assays, immunofluorescent labeling, and electron microscopy measured osteogenic cell responses to the HAp/PCL ://